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PCRFor microdissected cells, a volume of lysis buffer accounting for 500 cells was added in each PCR vial. For DNA extracted from blood cells, 10 DNA was used for each PCR. The following 10 microsatellite dinucleotide repeats were used (Table 2): D3S1573; D7S490; D8S1820; D8S261; D11S860; D11S1356; D13S171; D16S496; IGP53; and D17S855. The PCR final volume was 20 Thirty five cycles of PCR were performed. The analysis of the migration data were performed with Genescan 3.1 software (Applied Biosystems).
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